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. 2012 Feb;10(1):24–36. doi: 10.1089/adt.2011.423

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Copyright 2012, Mary Ann Liebert, Inc.

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Fig. 4. — Pilot screen against a panel of 26 known effectors. (A) and (B) The results are presented as a heatmap to show the performance of the panel of 26 effectors that prevent granule formation (green) or are cytotoxic (blue) or inactive in the assay (black). Cytotoxic and fluorescent compounds are highlighted. (C) Summary table of the panel of 26 effectors tested in the EGFR biosensor assay summary of IC₅₀ and EC₅₀ data for dose–response curves fitted using logistic four parameter sigmoid regression. In the EGFR kinase assay, IC₅₀ values were assessed following 10 min of pre-incubation of the compound with the enzyme (⁺) or 60 min (*). The standard error corresponds to the standard error of the regression. Cytotoxicity is assessed based on the nuclei count, and a compound was deemed cytotoxic if the imaged nuclei count was less than 50% of the DMSO control wells. 2,4-Thiazolidinedione is the abbreviated name for 3-(2-aminoethyl)-5-((4-ethoxyphenyl)methylene)-2,4-thiazolidinedione. NE, no effect; ND, not determined; NA, not applicable; PI3K, phosphatidylinositol-3-kinase; PDGFR, platelet-derived growth factor receptor; PKC, protein kinase C; VEGFR, vascular endothelial growth factor receptor.

Fig. 4. — Pilot screen against a panel of 26 known effectors. (A) and (B) The results are presented as a heatmap to show the performance of the panel of 26 effectors that prevent granule formation (green) or are cytotoxic (blue) or inactive in the assay (black). Cytotoxic and fluorescent compounds are highlighted. (C) Summary table of the panel of 26 effectors tested in the EGFR biosensor assay summary of IC₅₀ and EC₅₀ data for dose–response curves fitted using logistic four parameter sigmoid regression. In the EGFR kinase assay, IC₅₀ values were assessed following 10 min of pre-incubation of the compound with the enzyme (⁺) or 60 min (*). The standard error corresponds to the standard error of the regression. Cytotoxicity is assessed based on the nuclei count, and a compound was deemed cytotoxic if the imaged nuclei count was less than 50% of the DMSO control wells. 2,4-Thiazolidinedione is the abbreviated name for 3-(2-aminoethyl)-5-((4-ethoxyphenyl)methylene)-2,4-thiazolidinedione. NE, no effect; ND, not determined; NA, not applicable; PI3K, phosphatidylinositol-3-kinase; PDGFR, platelet-derived growth factor receptor; PKC, protein kinase C; VEGFR, vascular endothelial growth factor receptor.