Table 1.
The Comparison of Potency and Efficacy of Purinergic 2Y Agonists to Trigger Dynamic Mass Redistribution in Confluent HEK293 Cultured on Four Different Surfaces
| Surface |
ATP |
ATPγS |
2-MeSATP |
ADP |
UTP |
UTPγS |
UDP |
|---|---|---|---|---|---|---|---|
| (a) Potency (nM) | |||||||
| TCT | 1025±127 | 1145±93 | 31±4 | 84±6 | 2142±151 | 3327±325 | 113,000±13,000 |
| Fibronectin | 654±31 | 605±33 | 19±3 | 43±5 | 1076±97 | 2793±411 | 41,241±5639 |
| Collagen IV | 1322±192 | 1303±114 | 26±2 | 68±7 | 1364±159 | 3104±276 | 64,811±7100 |
| Gelatin | 973±74 | 1000±81 | 27±5 | 69±3 | 1818±198 | 3049±301 | 114,000±15,000 |
| (b) Efficacy (the peak response in picometer) | |||||||
|---|---|---|---|---|---|---|---|
| TCT | 213±21 | 148±9 | 98±7 | 106±10 | 149±11 | 174±13 | 154±15 |
| Fibronectin | 244±19 | 168±10 | 113±6 | 116±7 | 188±13 | 187±14 | 267±23 |
| Collagen IV | 256±23 | 154±9 | 84±5 | 99±5 | 160±12 | 193±11 | 265±21 |
| Gelatin | 242±24 | 175±11 | 123±11 | 128±6 | 178±12 | 210±19 | 106±13 |
2MeSATP, 2-methylthio-ATP; ADP, adenosine diphosphate; ATP, adenosine 5′-triphosphate; TCT, tissue culture-treated; UDP, uridine diphosphate; UTP, uridine triphosphate; UTPγS; uridine 5-thiotriphosphate.