Fig. 2.

Multi-parameter immunofluorescence visualization and quantitation of treated cells. (A) GR-GFP (green) was used to track cytoplasmic dynein translocation, a fluorescent tubulin antibody (red) to label MTs, and Hoechst 33342 was used to label DNA (blue) to observe the nuclei. (B) Mean circ-ring average intensity difference of GR-GFP content was measured to determine extent of GR translocation inhibition. (C) Mean mass of stained MTs by tubulin antibody provided information on cell morphology. (D) Mean number of cells was counted to evaluate cell viability after treatment with compounds. SD was calculated for DMSO and DEX from 14 and 16 independent experiments, respectively. SD for test agents was determined from three independent experiments. The statistical effect size for the assay was determined to be Z′=0.52±0.11. GR, glucocorticoid receptor; DEX, dexamethasone; DMSO, dimethylsulfoxide; GFP, green fluorescent protein; MT, microtubule; SD, standard deviation.