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. 2012 Feb;10(1):46–60. doi: 10.1089/adt.2010.0367

Fig. 3.

Fig. 3.

Fluorescence polarization GR competitive binding assay. (A) Concentration-dependent evaluation of the competitive binding of the GR agonist DEX fitted by a one-site competition curve with R2=0.9976 and an half maximal inhibitory concentration value of 10.1±1.5 nM. (B) Percent competition of test compounds against binding to GR normalized to DEX control. Data represented as mean values with SD from two independent experiments done in triplicate.