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. 2012 Feb;10(1):46–60. doi: 10.1089/adt.2010.0367

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Copyright 2012, Mary Ann Liebert, Inc.

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Fig. 5. — Cytoplasmic dynein ATPase inhibition assay. (A) Percent inhibition of ATPase activity of basal dynein with test compounds compared to DMSO control and sodium orthovanadate as a control. (B) Concentration-dependent inhibition of basal cytoplasmic dynein ATPase activities of (R)-apomorphine (R²=0.9943), (S)-apomorphine (R²=0.9918), 6-nitroso-1,2-benzopyrone (R²=0.9866), Bay 11–7805 (R²=0.9929). (C) Percent inhibition of ATPase activity of MT-stimulated dynein with test compounds compared to DMSO control and sodium orthovanadate as a control. (D) Concentration-dependent inhibition of MT-stimulated cytoplasmic dynein ATPase activities of (R)-apomorphine (R²=0.9973), (S)-apomorphine (R²=0.9937), 6-nitroso-1,2-benzopyrone (R²=0.9379), Bay 11–7805 (R²=0.9916). Data represented as mean values with SD from two independent experiments done in quadruplet.