Table 3.

Summary of Microtubule Polymerization Assay

Step	Parameter	Value	Description
1	Compound addition to plate	1 μL	1 mM compound in DMSO added to 96-well plates
2	Protein addition	80 μL	1.25 mg/mL tubulin in 1 M MSG, pH 6.6
3	Incubation time	15 min	On ice
4	GTP addition	19 μL	2.1 mM in assay buffer
5	Assay readout	340 nm	Absorbance–kinetic mode, 1 reading/min for 30 min at 37°C

4. GTP only added for MT destabilizer screen. MT stabilizer screen replaced with buffer only.

 GTP, guanosine triphosphate; MSG, monosodium glutamate; MT, microtubule.