Sample preparation workflows for the quantification of cardiovascular biomarkers in serum.
A, Sample preparation workflow using either depletion of abundant proteins or troponin I immunoenrichment before SDS-PAGE, in-gel trypsin digestion and LC-SRM analysis. B, Multiplex LC-SRM detection of LDH-B, CKB chain, CKM chain and myoglobin in a healthy donor serum sample after depletion of abundant proteins. For better clarity, a single SRM transition from one proteotypic peptide (labeled and unlabeled versions) is shown for each biomarker.