Table I. Peptide sequences and SRM transitions. Peptides from PSAQ standards are mentioned with the C-terminal isotope-labelled amino acid in bold. Methionine dioxidation (ox2) or cystein trioxidation (ox3) modification states are indicated.
| Biomarker | UniProt accession number | Peptide sequence | SRM transitions |
Collision energy (Volts) | |
|---|---|---|---|---|---|
| Q1 m/z | Q3 m/z | ||||
| LDH-B | P07195 | IVVVTAGVR | 457.3 | 503.3 | 27.9 |
| 457.3 | 602.4 | 27.9 | |||
| 457.3 | 701.4 | 27.9 | |||
| IVVVTAGVR | 462.3 | 513.3 | 27.9 | ||
| 462.3 | 612.4 | 27.9 | |||
| 462.3 | 711.4 | 27.9 | |||
| GLTSVINQK | 480.3 | 502.3 | 29.0 | ||
| 480.3 | 601.4 | 29.0 | |||
| 480.3 | 688.4 | 29.0 | |||
| GLTSVINQK | 484.3 | 510.3 | 29.0 | ||
| 484.3 | 609.4 | 29.0 | |||
| 484.3 | 696.4 | 29.0 | |||
| SLADELALVDVLEDK | 815.4 | 504.3 | 45.8 | ||
| 815.4 | 718.4 | 45.8 | |||
| 815.4 | 1001.6 | 45.8 | |||
| 815.4 | 1114.6 | 45.8 | |||
| 544.0 | 504.3 | 31.2 | |||
| 544.0 | 718.4 | 31.2 | |||
| 544.0 | 817.4 | 31.2 | |||
| SLADELALVDVLEDK | 819.4 | 512.3 | 45.8 | ||
| 819.4 | 726.4 | 45.8 | |||
| 819.4 | 1009.6 | 45.8 | |||
| 819.4 | 1122.6 | 45.8 | |||
| 546.6 | 512.3 | 31.2 | |||
| 546.6 | 726.4 | 31.2 | |||
| 546.6 | 825.4 | 31.2 | |||
| LIAPVAEEEATVPNNK | 848.0 | 472.3 | 47.4 | ||
| 848.0 | 1201.6 | 47.4 | |||
| LIAPVAEEEATVPNNK | 852.0 | 480.3 | 47.4 | ||
| 852.0 | 1209.6 | 47.4 | |||
| Total LDH: | P00338 | VIGSGC(ox3)NLDSAR | 620.3 | 333.2 | 36.0 |
| LDH-A, LDH-B and LDH-C | P07195 | 620.3 | 448.2 | 36.0 | |
| P07864 | 620.3 | 675.3 | 36.0 | ||
| VIGSGC(ox3)NLDSAR | 625.3 | 343.2 | 36.0 | ||
| 625.3 | 458.2 | 36.0 | |||
| 625.3 | 685.3 | 36.0 | |||
| Creatine kinase B | P12277 | DLFDPIIEDR | 616.8 | 742.4 | 35.8 |
| 616.8 | 857.4 | 35.8 | |||
| DLFDPIIEDR | 621.8 | 752.4 | 35.8 | ||
| 621.8 | 867.4 | 35.8 | |||
| VLTPELYAELR | 652.4 | 990.5 | 37.6 | ||
| 652.4 | 1091.6 | 37.6 | |||
| VLTPELYAELR | 657.4 | 1000.5 | 37.6 | ||
| 657.4 | 1101.6 | 37.6 | |||
| Creatine kinase M | P06732 | FEEILTR | 454.3 | 502.3 | 27.7 |
| 454.3 | 631.4 | 27.7 | |||
| FEEILTR | 459.3 | 512.3 | 27.7 | ||
| 459.3 | 641.4 | 27.7 | |||
| ELFDPIISDR | 602.8 | 700.4 | 35.1 | ||
| 602.8 | 962.5 | 35.1 | |||
| ELFDPIISDR | 607.8 | 710.4 | 35.1 | ||
| 607.8 | 972.5 | 35.1 | |||
| GGDDLDPNYVLSSR | 754.4 | 935.5 | 42.7 | ||
| 754.4 | 1050.5 | 42.7 | |||
| GGDDLDPNYVLSSR | 759.4 | 945.5 | 42.7 | ||
| 759.4 | 1060.5 | 42.7 | |||
| Myoglobin | P02144 | VEADIPGHGQEVLIR | 545.0 | 553.3 | 31.2 |
| 545.0 | 702.9 | 31.2 | |||
| VEADIPGHGQEVLIR | 548.3 | 558.3 | 31.2 | ||
| 548.3 | 707.9 | 31.2 | |||
| Troponin I | P19429 | TLLLQIAK | 450.3 | 685.5 | 27.5 |
| 450.3 | 572.4 | 27.5 | |||
| TLLLQIAK | 454.3 | 693.5 | 27.5 | ||
| 454.3 | 580.4 | 27.5 | |||
| NITEIADLTQK | 623.3 | 675.4 | 36.2 | ||
| 623.3 | 1018.5 | 36.2 | |||
| NITEIADLTQK | 627.3 | 683.4 | 36.2 | ||
| 627.3 | 1026.5 | 36.2 | |||
| ISADAM(ox2)M(ox2)QALLGAR | 756.4 | 303.2 | 42.8 | ||
| 756.4 | 416.3 | 42.8 | |||
| 756.4 | 529.4 | 42.8 | |||
| ISADAM(ox2)M(ox2)QALLGAR | 761.4 | 313.2 | 42.8 | ||
| 761.4 | 426.3 | 42.8 | |||
| 761.4 | 539.4 | 42.8 | |||