Table II. PKA-mediated phosphorylation: semi-quantitative comparison of native and CuOOH-inactivated CYP3A4.
No. | Residue | Control | Native 3A4 (%) | CuOOH-3A4 (%) |
---|---|---|---|---|
1 | Ser-116 | — | 0.20 ± 0.05 | 0.27 ± 0.08 |
2 | Ser-119 | — | 0.01 ± 0.004 | 0.42 ± 0.06 |
3 | Ser-134 | — | 0.04 ± 0.01 | 0.06 ± 0.03 |
4 | Ser-259 | — | — | 0.49 ± 0.05 |
5 | Ser-478 | — | 0.32 ± 0.07 | 1.29 ± 0.12 |