Fig. 2.

PTRF expression affects multiple secretory pathways to reduce a subset of secreted proteins that function in cell adhesion and cytoskeleton remodeling. A, Bar chart of GeneGo analysis displaying the most highly enriched molecular pathways for the secretome and prostasome fraction. B, Venn diagrams displaying proteins common to the secretome and prostasome fraction (top) and those common in the list of proteins significantly changed (p ≤ 0.05) upon PTRF expression (bottom). C, PC-3 cells were transiently transfected with GFP or GFP-PTRF. The secretome was collected from serum free media incubated on the cells for 24 h. Whole cells lysates were isolated in parallel. Western blotting was performed to analyze changes in protein expression by probing with antibodies against PTRF, caveolin-1, KLK-6, and IL-6. Equal loading was shown by Coomassie stain. Whole cells lysates showed no difference in protein levels between GFP and GFP-PTRF cells however, secreted levels of KLK-6 and IL-6 were reduced in GFP-PTRF cells.