Fig. 7.

Cholesterol dynamics modulates secretion. The secretome was isolated from PC-3 cells incubated with serum free media for 24 h. During the final 6 h of incubation, cells were supplemented with vehicle (control), 8 μm cholesterol, or 500 μm MβCD. Changes in secretion were analyzed by Western blot analysis. Antibodies against β-actin, caveolin-1, and KLK-6 were used to detect secreted protein levels. Equal loading was shown by coomassie stain.