Fig. 1.

Characterization of cell lines expressing EGFP-tagged SIRT7 derivatives. A, diagram of three SIRT7 derivatives tagged with EGFP (dark green) and FLAG (dark blue)—wild type SIRT7 (WT), SIRT7 S111A mutant, containing a Ser to Ala mutation in the conserved GAGIST motif within the core domain (turquoise), and SIRT7 dE2 with deleted exon 2 (E2, light brown). B, immunoblotting with anti-GFP and anti-SIRT7 antibodies confirms the expression and approximate molecular masses of EGFP-tagged SIRT7 variants. C, the three EGFP-tagged SIRT7 variants have predominant nucleolar localizations and co-localize with nucleolar protein UBF, as confirmed by immunofluorescence using anti-UBF and anti-GFP antibodies in HEK293 cells. A 60× oil immersion lens was used. Endo. SIRT7, endogenous SIRT7. Bar, 10 μm.