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AS patient plasma can immunoprecipitate IL-6. 1, 10, and 100 ng of recombinant IL-6 was used as bait and immunoprecipitated with IgG molecules from 50 μl of pooled AS patient or control plasma samples. Immunoprecipitated (IP) material was separated by 16% SDS-PAGE and detected by anti-IL-6 immunoblotting.
