Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescence intensity decay of the 5-FAM/Cy5 peptide (1μM) measured with 470nm excitation and 520nm (5-FAM band) observation. The fluorescence of 5-FAM is strongly quenched and the decay is very heterogeneous. After the cleavage reaction the lifetime is long (3.4 ns) and homogeneous.
