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. Author manuscript; available in PMC: 2012 Feb 12.
Published in final edited form as: Nat Chem Biol. 2010 Jan 17;6(3):225–230. doi: 10.1038/nchembio.306

Figure 4. Nonnative interactions outside of the Sc4 amyloid core drive oligomer formation of Sup35NM.

Figure 4

(a,b) Normalized excimer fluorescence spectra of pyrene-labeled Sup35NM mutants at 4°C (a) or 37°C (b). Shown are fluorescence spectra of Cys16 (red), Cys89 (blue), Cys108 (black), and Cys238 (pink) mutants.

c) The ratio of excimer fluorescence intensities (470nm) to non-excimer fluorescence intensities (385 nm). Normalized intensities of excimer fluorescence of the pyrene-labeled Sup35NM mutants at 4°C (blue) or 37°C (red) were plotted against amino acid position.

(d) Normalized intensities of excimer fluorescence are plotted against temperature for pyrene-labeled Sup35NM Cys16 (red), Cys26 (orange), Cys46 (yellow), Cys89 (blue) and Cys108 (black) mutants.