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Localization of EccCbms is dependent on other ESX-1-associated proteins. In an M. smegmatis Δesx1 mutant, (A), or a ΔeccCb-esxA mutant (B), EccCbms-YFP no longer assembled at the cell pole and appeared diffuse throughout the cell. Expression of ESX-1mt completely restores polar localization of EccCbms-YFP in the absence of ESX-1ms (C).
