Figure 1.
Ad-mediated gene transfer and expression in DCs in vitro. AGR2 gene was cloned and monocyte-derived DCs were infected with AdAGR2 at a multiplicity of infection (MOI) of 1,000. Seventy-two hours later, the expression of AGR2 was analyzed by RT-PCR (A) and WB (B). HCT-8 and HCT-116 cells were used as AGR2-positive and -negative controls, respectively.