Figure 3.
AGR2-specific CTL response by transduced DCs. ELISPOT assay showing the number of IFN-γ-secreting cells per 105 CD8+ T cells induced by AdGFP- and AdAGR2-transduced DCs, respectively, at an E:T ratio of 20:1. Several AGR2-expressing CRC cell lines were used as targets. MHC class I restriction of the CTLs was analyzed using a MHC class I specific monoclonal Ab (clone W6/32). Nonspecific response was determined by irrelevant Ig blockade. Representative results of 4 independent experiments are shown.