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. 1979 Apr;6(4):1571–1581. doi: 10.1093/nar/6.4.1571

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.

P Davanloo, M Sprinzl, K Watanabe, M Albani, H Kersten
PMCID: PMC327791  PMID: 377228

Abstract

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.

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Selected References

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