FIG. 11.

Analyses of cell markers during activation of naive CD4⁺ T cells from mice fed different Se diets. Under conditions previously described (102), purified splenic CD4⁺ T cells were stimulated for 18 h through the TCR, and flow cytometry was used to measure markers for Th1 cells (CD40L), T_reg cells (CD25 and FoxP3), and a marker excluded from T_reg cells (RANKL). Preliminary studies in our laboratory suggest that increased Se intake leads to higher levels of Th1 and T_reg markers. FoxP3, forkhead box P3; RANKL, receptor activator for nuclear factor-κB ligand.