Figure 4.

Contacts between CGRP-containing or CTb-labeled primary afferents and dendrites of large gephyrin-coated lamina I cells in horizontal sections. a–d, Confocal images scanned to reveal gephyrin (red), CGRP (green), and NF200 (blue) show part of a dendrite that is coated with gephyrin, contains NF200, and receives a few contacts from CGRP-immunoreactive boutons (3 of which are labeled with arrowheads). e–h were scanned to reveal gephyrin (red), CTb (green), and NF200 (blue) from a rat in which CTb had been injected into the sciatic nerve 3 d previously. Part of a dendrite that contains NF200 and is coated with gephyrin puncta is shown, and this receives contacts from CTb-labeled varicosities (2 shown with arrowheads). All images are projections from four optical sections at 0.5 μm z-separation. Scale bar, 10 μm.