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. 2012 Feb 13;3:11. doi: 10.3389/fphar.2012.00011

Figure 1.

Figure 1

CyPPA inhibits spontaneous activity via. prolongation of the apamin-sensitive medium afterhyperpolarization (mAHP) in SNc DA neurons in mice midbrain slices. (A) Typical pacemaker-like activity (black trace), is inhibited by superfusion with 10 μM CyPPA (blue trace), which increases mAHPs and eventually silences firing activity. Co-administration with 10 μM NS8593 resumes pacemaker firing (green trace), whereas apamin (300 nM) caused a slightly further increase in firing frequency and more irregular firing. (B) Concentration-response curve of spontaneous firing activity before and after CyPPA superfusion (control, 1, 3, 10 μM, n = 7, 2, 3, and 5 respectively. One-way ANOVA followed by Holm Sidak multiple comparison test. *P < 0.05 and ***P < 0.001). (C) Overlain single spikes before and after CyPPA (10 μM), CyPPA + NS8593 (10 μM), and CyPPA + NS8593 + apamin (300 nM). Color coding as in (A). Note the prolongation of the mAHP by CyPPA, which is reduced by NS8593, and completely blocked by apamin.