At low concentrations,
AHA exposure is not toxic and does not significantly
alter simple behaviors. (a) Survival rate of 7-day-old larval zebrafish
when incubated with AHA (0–20 mM, 6–72 h) or methionine
(10 mM, 6–72 h), n = 20. (b) Quantification
of spontaneous swimming behavior of larval zebrafish after AHA incubation
(4 mM, 0–48 h). Percentage of larvae that show no spontaneous
swimming behavior per 15 min interval. Mean swimming bursts per 15
min interval, n = 10–12. Differences are not
statistically significant. (c) Traces depicting the angle of eye rotation
during a typical optokinetic response after AHA incubation (4 mM,
0–48 h). (d) Sample startle response upon light flash after
AHA incubation (4 mM, 24 h). (e) Mean response percentage to light
or dark flash after AHA incubation (4 mM, 0–48 h), n = 5 larvae, flashed three times each. Error bars represent
standard deviation of response percentage. Differences are not statistically
significant. (f) Mean delay in response to light or dark flash after
AHA incubation (4 mM, 0–48 h), n = 5 larvae,
flashed three times each. Error bars represent standard deviation
of response time. Differences are not statistically significant.