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. 2011 Nov 7;3(1):40–49. doi: 10.1021/cn2000876

Figure 2.

Figure 2

At low concentrations, AHA exposure is not toxic and does not significantly alter simple behaviors. (a) Survival rate of 7-day-old larval zebrafish when incubated with AHA (0–20 mM, 6–72 h) or methionine (10 mM, 6–72 h), n = 20. (b) Quantification of spontaneous swimming behavior of larval zebrafish after AHA incubation (4 mM, 0–48 h). Percentage of larvae that show no spontaneous swimming behavior per 15 min interval. Mean swimming bursts per 15 min interval, n = 10–12. Differences are not statistically significant. (c) Traces depicting the angle of eye rotation during a typical optokinetic response after AHA incubation (4 mM, 0–48 h). (d) Sample startle response upon light flash after AHA incubation (4 mM, 24 h). (e) Mean response percentage to light or dark flash after AHA incubation (4 mM, 0–48 h), n = 5 larvae, flashed three times each. Error bars represent standard deviation of response percentage. Differences are not statistically significant. (f) Mean delay in response to light or dark flash after AHA incubation (4 mM, 0–48 h), n = 5 larvae, flashed three times each. Error bars represent standard deviation of response time. Differences are not statistically significant.