Figure 3.

AHA is metabolically incorporated into larval zebrafish proteins in vivo. Sample immunoblot and quantification of immunoblots of lysates from AHA-treated 7-day-old larval zebrafish reacted with biotin-alkyne (10 μM) for 12 h, probed with antibody against biotin. (a) Larval zebrafish were incubated with 4 mM AHA for 0–72 h, n = 4. (b) Larval zebrafish were incubated with AHA (0 or 4 mM) or 4 mM AHA in the presence of puromycin (2.5–10 μg/mL) for 48 h, n = 3. ***p < 0.001.