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. 2011 Nov 7;3(1):40–49. doi: 10.1021/cn2000876

Figure 5.

Figure 5

GABA antagonist PTZ induces increased protein synthesis in larval zebrafish. (a) Sample immunoblot and quantification of immunoblots of lysates from 7-day-old larval zebrafish reacted with biotin-alkyne tag (10 μM) for 12 h, probed with antibody against biotin. Zebrafish were incubated with 4 mM AHA (0 or 30 h) or with 4 mM AHA for 30 h as well as 15 mM PTZ for two periods of 2 h, 20 and 6 h before harvesting, n = 3. ***p < 0.001. (b) Western blot of biotin affinity-purified lysates of zebrafish incubated with 4 mM AHA for 30 h with or without 4 h 15 mM PTZ exposure. (c) Imaging of 7-day-old larval zebrafish after 48 h 4 mM AHA incubation with or without 4 h 15 mM PTZ exposure, reacted with AlexaFluor-488-alkyne (5 μM, 12 h); dorsal view. Scale bar is 150 μm; n = 6.