GABA antagonist PTZ induces increased protein synthesis
in larval
zebrafish. (a) Sample immunoblot and quantification of immunoblots
of lysates from 7-day-old larval zebrafish reacted with biotin-alkyne
tag (10 μM) for 12 h, probed with antibody against biotin. Zebrafish
were incubated with 4 mM AHA (0 or 30 h) or with 4 mM AHA for 30 h
as well as 15 mM PTZ for two periods of 2 h, 20 and 6 h before harvesting, n = 3. ***p < 0.001. (b) Western blot
of biotin affinity-purified lysates of zebrafish incubated with 4
mM AHA for 30 h with or without 4 h 15 mM PTZ exposure. (c) Imaging
of 7-day-old larval zebrafish after 48 h 4 mM AHA incubation with
or without 4 h 15 mM PTZ exposure, reacted with AlexaFluor-488-alkyne
(5 μM, 12 h); dorsal view. Scale bar is 150 μm; n = 6.