Table 1.
Comparisons of Rg from continuous-flow SAXS.a
| Proteinb | SAXS Rgc | Dead time | ||
|---|---|---|---|---|
| Unfolded (Å) | Continuous-flow burst phase (Å) | Native (Å) | ||
| Ub42 | 26 | 25.93 ± 0.03 | 13.9 | 2.5 ms |
| ctAcP42 | 31 | 28.41 ± 0.23 | 14.6 | 2.5 ms |
| DHFR30 | 30.7 | 23.2 ± 0.3 | 16.6 ± 0.1 | 300 μs |
| αTS31 | 43 | 34 | 18.1 | 150 μs |
| cyt c26 | 24 | 20.5 ± 1 | 13.9 | 160 μs |
| apoMb51 | 29.7 ± 1.7 | 23.7 ± 0.9 | 18.2 ± 0.2 | 300 μs |
| SMN53 | 25.5 ± 0.5 | 18.2 ± 0.4 | 15.8 ± 0.2 | 300 μs |
| HO52 | 37.8 ± 1.2 | 26.1 ± 1.1 | 23 ± 1.2 | 600 μs |
a
Radii of gyration of the burst-phases of different proteins observed by continuous-flow refolding kinetics. The sizes of the native and unfolded states are also given for reference. The dead-time varies between 150 μs and 2.5 ms and is given for each protein.
b
Ub, ubiquitin; ctAcP, common type acyl-phosphatase; DHFR, E.coli dihydrofolate reductase; αTS, α subunit of tryptophan synthase; cyt c, horse heart cytochrome c; apoMb, apomyoglobin; SMN, single-chain monellin; HO, heme-oxygenase.
c
When not explicitly given, estimates of the errors for unfolded and continuous-flow burst phase Rg are approximately ±1 Å and those of the native state are approximately ±0.2 Å.