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. 2012 Jan 18;95(3):670–677. doi: 10.3945/ajcn.111.026708

FIGURE 1.

FIGURE 1.

The mean (±SD) relative affinity of G12 moAb to immunotoxic peptides derived from PWG gliadin after simulated gastrointestinal digestion. A and B: SDS-PAGE and Western blot of PWG gliadin, PWG gliadin + pepsin, and PWG gliadin + pepsin + trypsin/chymotrypsin. Samples were stained with silver (A) or after transfer to a polyvinylidene fluoride membrane and incubated with G12 moAbs (B). C: Competition assay that measured the affinity of the horseradish peroxidase–conjugated G12 moAb to gliadin peptides present in gastrointestinal digests. Three assays were performed with samples run in triplicate. moAb, monoclonal antibody; MW, molecular weight marker; PWG, Prolamin Working Group.