FIGURE 4.

Detection of gluten in feces of healthy individuals subjected to a controlled gluten-containing diet. A: Semiquantitative analysis of gluten peptides and proteins in feces of healthy individuals by using G12 immunochromatographic strips HL901–HL911 (studied subjects: n = 11). An asterisk indicates that gluten traces were detected. B: One representative immunochromatographic-strip example of the trial performed with the samples collected during the study period for one subject. Blue stripes represent an internal positive control that indicates that the stick worked properly; pink stripes indicate the presence of gluten. C and D: SDS-PAGE and Western blot of gluten peptides and proteins extracted from feces. GFD, gluten-free diet; MW, molecular weight marker; PWG, Prolamin Working Group.