Figure 4.

Nephrin and PC immunodetection. (A) nephrin expression (white arrows) in control rats; (B) sStx2-treated rats showing a decreased expression of nephrin (white arrowhead); (C) PC expression in control rats (white arrowhead); (D) a marked decreased expression of PC in sStx2-treated rats. The number of positive marks of nephrin and PC was counted in ten fields of 200× using a grid superimposed on the image. (E) Expressions of nephrin (176.33 ± 16.6 vs 32.16 ± 10.6; P < 0.01, n = 6) and (F) PC (122.16 ± 10.6 vs 19.16 ± 7.9; P < 0.01, n = 6) were significantly decreased with respect to the controls.

Note: Magnification for (A–D) at 200×.

Abbreviations: sStx2, supernatant Shiga toxin type 2; PC, podocalyxin.