. Author manuscript; available in PMC: 2012 Feb 13.

Published in final edited form as: Nat Cell Biol. 2011 Feb 20;13(3):203–214. doi: 10.1038/ncb2163

Table 1.

Most early transcriptional changes in basal cells depleted of Srf involve regulators of actomyosin and not cell cycle, inflammation, ABP or Notch signalling genes.

Actomyosin	Fblim1(−3.7)^* Cnn2(−3.1)^* Actb(−2.0)^* Flna(−1.8)^* Arhgap24(−1.8)^* Actg1(−1.7)^* Wdr1(−1.6)^* Pfn2(−1.3)^* Gsn(−1.3)^*
cytoskeleton	Myh9^† Myh10^† Myh14^† Myl9^†
Cell adhesion	Gjb5(−2.6)^* Pcad20(−2.2)^* Dsg1b(−1.8)^* Tns4(−1.6)^* Cdh1^† Ctnna1^† Ctnnb1^† Tjp1^† Tjp2^† Ocln^† Itgb1^†
Immediate early genes	Egr2(−8.6)^* Egr1(−5.0)^* Egr3(−3.9)^* Fos^† Junb^†
Immune response	Il1b^† Il6^† Il18^† Ccl2^† Ccl27a^† S100a8^† S100a9^†
Cell cycle	Mki67^† Ccna1^† Ccna2^† Ccnb1^† Ccnb2^† Ccnd1^† Ccnd2(1.5)^‡ Ccnd3^† Ccne1^† Ccne2^†
Notch signalling	Notch1^† Notch2^† Notch3^† Jag1^† Jag2 ^† Dll1^†
Apico-basal	Pard3^† Pard3b^† Pard6a^† Pard6b^† Pard6g ^† Prkcc^† Prkcz^†
polarity

Embryos were from K14–Cre×Rosa26YFP fl/fl ×Srf fl/fl (wild-type and cKO littermates). Basal cells active for Cre recombinase expressed YFP and were enriched for surface α6-integrin. After FACS purification, mRNAs were subjected in duplicate for microarray analyses. Data from two independent arrays revealed 72 mRNAs that scored as up- or downregulated by at least 1.5-fold with P <0.05. DAVID clustering analyses showed that the affected process is the actomyosin cytoskeleton, whereas ABP, Notch, cell cycle regulators and pro-inflammation associated genes showed no change at this early time.

Decrease in early (E16.5) mRNA expression

^†

No change in early (E16.5) mRNA expression

^‡

Increase in early (E16.5) mRNA expression