Table 2.
E coli | pH | XR (U/gCDW) | FDH (U/gCDW) | Substrate (mM) | Yield (mM)1 | Yield (mM)1, 2 |
---|---|---|---|---|---|---|
BL21_XR_FDH | 7.5 | 1406 | 110 | 100 | 19 | 46 |
Rosetta_XR_2FDH | 7.5 | 1140 | 251 | 100 | 35 | 67 |
BL21_XR_FDH | 6.2 | 2226 | 48 | 100 | 27 | 78 |
Rosetta_XR_2FDH | 6.2 | 2024 | 189 | 100 | 63 | 95 |
1Determined for a reaction time of 8 h. 2Additives: 36 μM Polymyxin B sulphate and 500 μM NAD+.