Table 3.
E. coli expression strains addressed in this study
| E. coli host | Plasmid | Enzyme | Antibiotic | Abbreviation |
|---|---|---|---|---|
| JM109 | pBTac1 | CbFDH | Amp1 | JM109_FDH [8] |
| BL21 (DE3) | pRSF-1b | CbFDH | Kan2 | BL21_FDH |
| Rosetta 2 (DE3) | pRSF-1b | CbFDH | Cam3, Kan | Rosetta_FDH |
| BL21 star (DE3) | pRSF-1b | CbFDH | Kan | Star_FDH |
| BL21 (DE3) | pETDuet-1 | CtXR, CbFDH | Amp | BL21_XR_FDH |
| BL21 (DE3) | pETDuet-1 pRSF-1b |
CtXR, CbFDH; CbFDH | Amp, Kan | BL21_XR_2FDH |
| Rosetta 2 (DE3) | pETDuet-1 | CtXR, CbFDH | Cam, Amp | Rosetta_XR_FDH |
| Rosetta 2 (DE3) | pETDuet-1 pRSF-1b |
CtXR, CbFDH; CbFDH | Cam, Amp, Kan | Rosetta_XR_2FDH |
| BL21 star (DE3) | pETDuet-1 pRSF-1b |
CtXR, CbFDH; CbFDH | Amp, Kan | Star_XR_2FDH |
1 Ampicillin 115 mg/L; 2 kanamycin 50 mg/L; 3 chloramphenicol 34 mg/L.