Figure 1.

Immunohistochemical identification of target neurons. (a+b) Pigmented vs. non-pigmented catecholaminergic neurons. TH⁺ neurons were identified by their brown cytosolic reaction product. Pigmented (TH⁺/NM⁺; top left) and non-pigmented (TH⁺/NM^-; bottom right, marked for LMD) neurons were further distinguished by the visible presence or absence of NM. (a) TH⁺/NM^- neuron before and (b) after LMD. (c+d) Catecholaminergic vs. non-catecholaminergic neurons. NeuN⁺ immunoreactivity results in a grey appearance of TH^- neurons due to a DAB/Nickel reaction (bottom neuron). (c) TH⁺/NM⁺ neuron before and (d) after LMD.