Figure 1.

Magel2 represses Clock:Bmal1-mediated transcription at an E-box promoter. Dual luciferase transcription assays following transfection of NIH3T3 cells with the mPer2:luc reporter plasmid and a renilla internal control plasmid, together with combinations of expression plasmids variously encoding epitope-tagged Clock, Bmal1, Magel2, Cry1, and Per2 proteins. Data are normalized to the baseline activity of the normalized mPer2:luc reporter. A * indicates a significant difference from the baseline mPer2:luc activity, and a # indicates a significant repression compared to Bmal1:Clock-mediated activation (p < 0.05). These results represent one of three experiments completed in triplicate. Error bars represent the standard error of the mean.