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. Author manuscript; available in PMC: 2012 Feb 13.
Published in final edited form as: J Neuroimmunol. 2011 Nov 12;240-241:129–136. doi: 10.1016/j.jneuroim.2011.10.009

Fig. 2.

Fig. 2

IEF immunoblots reveal phage peptide recognition of persistent antibodies in MS CSF. Paired MS CSF (C1 and C2) and an inflammatory control patient with chronic progressive meningoencephalitis (IC) CSF (3–5 μg total IgG) were resolved on agarose IEF gels and transferred to nitrocellulose membranes. Blots were probed with corresponding phage peptides (1010 pfu/ml) and incubated with mouse anti-pIII antibody followed by AP-anti-mouse IgG. Phage peptides recognized multiple IgG bands in the paired MS CSF and revealed additional bands in the second CSF, but not in IC CSF. Positive controls were CSF (100 ng of total IgG) probed with AP-anti-human IgG. Experiments were repeated three times. A, patient 1 (02-24); B, patient 2 (04-3); C, patient 3 (05-4). Data represent at least three independent experiments.