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. Author manuscript; available in PMC: 2012 Feb 13.
Published in final edited form as: J Neuroimmunol. 2011 Nov 12;240-241:129–136. doi: 10.1016/j.jneuroim.2011.10.009

Fig. 3.

Fig. 3

Persistent peptide antigens in MS CSF represent linear epitopes. Phage peptides selected by MS CSF IgG (two peptides from patients 1 and 3, and one from patient 2) were separated on gradient 4–12% SDS-polyacrylamide gels, blotted to PVDF membranes and probed with corresponding CSF obtained at two time intervals (CSF #1 and CSF #2), followed by HRP-conjugated anti-human IgG detection. Duplicate blots were probed with anti-pIII antibody to detect phage minor coat protein as positive controls. In each patient, phage peptides were recognized by the paired CSF, indicating that they represent linear epitopes. The anti-pIII antibody blots showed a 64-kDa band of phage minor coat protein with all phage peptides. Data represent at least three independent experiments.