Figure 4. Effect of hypoxic conditions, EGLN inhibitors or HIF-1α deficiency on TR1 activity.

(A) TR1 activity was measured as indicated in Methods in EMT6 and DT cells grown under normoxic (Nx) or hypoxic (Hx) conditions for 12 h. (B) EMT6 and DT cells were treated with 150 µM deferoxamine (DFX) or left untreated (Ctrl) for 12 h and TR1 activity measured. (C) EMT6 and DT cells infected with retrovirus encoding a scramble sequence (SCR) or a HIF-1α shRNA (si HIF-1α) were cultured under normoxic (Nx) or hypoxic (Hx) conditions for 12 h and TR1 activity measured. Data represent the means of 3 independent experiments ± SE.