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. 2012 Feb 13;7(2):e31205. doi: 10.1371/journal.pone.0031205

Table 1. Expression of P1 and P2 receptors, and ectonucleotidases, as determined by RT-PCR.

Genes Resident M1 M2
P2rx1
P2rx2
P2rx3
P2rx4 + + +
P2rx5
P2rx6
P2rx7 + + +
P2ry1 + + +
P2ry2 + + +
P2ry4
P2ry6 ± ± ±
P2ry12 ± ± ±
P2ry13
P2ry14 + + +
Adora1 (A1) ± ± ±
Adora2a (A2A) + + +
Adora2b (A2B) ± ± ±
Adora3 (A3) + + +
Entpd1 + + +
Entpd2 ± ± ±
Entpd3 ± ± ±
Entpd8
Nt5e + + +

The table shows gene/β-actin ratio determined after electrophoresis on a 1.5% agarose gel stained with SyberGold and visualization under ultraviolet light by using ImageJ 1.37 for Windows. Low DNA Mass Ladder (Invitrogen) was used as a molecular marker. The genes with gene/β-actin ratio ≥0.7 were considered strong expression (+), genes with ratio ≤0.3 were considered as barely detectable (±) while genes without any signal detected were considered as negative (−).