Table 1. Expression of P1 and P2 receptors, and ectonucleotidases, as determined by RT-PCR.
Genes | Resident | M1 | M2 |
P2rx1 | − | − | − |
P2rx2 | − | − | − |
P2rx3 | − | − | − |
P2rx4 | + | + | + |
P2rx5 | − | − | − |
P2rx6 | − | − | − |
P2rx7 | + | + | + |
P2ry1 | + | + | + |
P2ry2 | + | + | + |
P2ry4 | − | − | − |
P2ry6 | ± | ± | ± |
P2ry12 | ± | ± | ± |
P2ry13 | − | − | − |
P2ry14 | + | + | + |
Adora1 (A1) | ± | ± | ± |
Adora2a (A2A) | + | + | + |
Adora2b (A2B) | ± | ± | ± |
Adora3 (A3) | + | + | + |
Entpd1 | + | + | + |
Entpd2 | ± | ± | ± |
Entpd3 | ± | ± | ± |
Entpd8 | − | − | − |
Nt5e | + | + | + |
The table shows gene/β-actin ratio determined after electrophoresis on a 1.5% agarose gel stained with SyberGold and visualization under ultraviolet light by using ImageJ 1.37 for Windows. Low DNA Mass Ladder (Invitrogen) was used as a molecular marker. The genes with gene/β-actin ratio ≥0.7 were considered strong expression (+), genes with ratio ≤0.3 were considered as barely detectable (±) while genes without any signal detected were considered as negative (−).