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. 2012 Feb 13;7(2):e31148. doi: 10.1371/journal.pone.0031148

Figure 4. CD4+127+132− T-cells are enriched in Cord Blood mononuclear cells and contain a high proportion of sjTRECS compared with CD127+132+ and CD127−132+ T-cells.

Figure 4

a–b) CD4+127+132− T-cells are enriched for sjTRECS in healthy adult volunteers and patients with primary HIV infection. Cryopreserved PBMCs from healthy volunteers (N = 4) or patients with primary HIV infection (PHI; N = 4) were sorted into CD4+127+132−, CD4+127+132+ or CD4+127−132+ populations. DNA was then extracted and the TREC: C-α ratio was determined by real-time PCR as shown in the box-plots below. b) Additional proof of principle experiments confirm the higher concentration of TRECs in naïve CD4+ T-cells as compared with memory T-cells as shown in bar charts below. *p<0.05; **p<0.01 as measured by the Kruskal Wallis non-parametric test. Cord Blood mononuclear cells had a high concentration of TRECS that were not enriched in the CD127+132− subset again as shown in bar charts. c) Cord blood mononuclear cells have a trend towards a greater proportion of CD127+132− CD4+ T-cells and a smaller proportion of CD127−132+ CD4+ T-cells as compared with adult PBMCs. Data representative of 10 subjects.