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. 2012 Feb 13;7(2):e31279. doi: 10.1371/journal.pone.0031279

Figure 8. Adeno-ILK prevents apoptosis, and promotes proliferation, of cultured neonatal rat cardiomyocytes treated with doxorubicin.

Figure 8

A, Effect of doxorubicin on cell viability, as assessed by MTT assay, and the effect of ILK on this. n = 6 per group. *, **P<0.05 and <0.01 respectively. B, Effect of doxorubicin on cardiomyocyte apoptosis, as assessed by annexin V expression (determined by flow cytometry), and the effect of ILK on this. Necrotic cells were excluded from the analysis by gating on PI-negative cells. n = 6 per group. *, **P<0.05 and <0.01 respectively. C, Effect of ILK on proliferation of neonatal rat cardiomyocytes treated with doxorubicin, as assessed by phosphohistone-H3 staining (brown); this showed more positive nuclei (arrow) in the adeno-ILK group than the adeno-null group. Graph shows accumulated results of n = 6 experiments. **P<0.01 versus adeno-null group. Scale bar, 100 µm.