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. 2012 Feb 13;7(2):e31554. doi: 10.1371/journal.pone.0031554

Figure 9. Age-dependent increases in CaMKII binding to the N-terminal domain of spinophilin.

Figure 9

A. Whole forebrain TSFs were incubated with GST, GSTSpN, GSTSpN1, or GSTSpN2. Complexes were isolated and immunoblotted for both CaMKIIα and CaMKIIβ. B. Striatal TSFs isolated from PND21 (P21) and adult animals were incubated with GSTSpN1 or GSTSpN2. Complexes were isolated using glutathione agarose and immunoblotted for CaMKIIα. C. Immunoblot of α-internexin from striatal TSFs isolated from PND21 and adult animals. D. Striatal TSFs isolated from PND21 (P21) and adult animals were incubated with GSTSpN1. Complexes were isolated and immunoblotted for myosin Va or α-internexin. All values were normalized to the mean at PND21, and then expressed as the mean±S.E.M from the indicated number of animals. * P<0.05, **P<0.01.