Table 1. Design of gene targeting for dArf6, Dscam-N and Dscam-C founder Knock-out lines.
Target Gene | Target Chromo-some | Exons/mRNA Isoforms | 5′+3′ Arms* (kb) | Targeted gDNA Deletion** | Genomic Deletion Size (kb) | Protein Deletion/Full Length (aa) |
dArf6 | 2nd | 3/5 | 4.5+3.1 | 2R: 11,210,875–11,213,032 | 2.157 | 175/175 |
Dscam-N | 2nd | 24/38016 | 5.5+3.2 | 2R:3249024—3254750 | 5.727 | 108/2037 |
Dscam-C | 2nd | 24/38016 | 5.3+3.2 | 2R:3206840—3214484 | 7.645 | 439/2037 |
*: 5′+3′ Arms: the lengths of 5′ and 3′ homology arms in targeting construct.
**: According to Drosophila genome release FB2011.07 at www.flybase.org.