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. Author manuscript; available in PMC: 2013 Mar 10.
Published in final edited form as: Gene. 2012 Jan 5;495(2):95–103. doi: 10.1016/j.gene.2011.12.057

Fig 1. mRNA levels of Col10a1 and p53 family members in MCT cells.

Fig 1

(A). Total RNAs were prepared from both proliferative (32 °C) and hypertrophic (37 °C) MCT cells using the TRIzol reagent. Reverse transcribed cDNA were subjected to RT-PCR analysis and the results showed that Col10a1, p53 and p63 transcripts were much more abundant in hypertrophic MCT cells than in proliferative MCT cells. No p73 amplicon was detected using the designated pair of PCR primers. (B). Real-time RT-PCR analysis showed that Col10a1 mRNA transcript is more than 20-fold upregulated in hypertrophic MCT cells than in proliferative MCT cells as normalized to Gapdh. Meanwhile, both p53 and p63 showed 2–3 fold upregulation in hypertrophic MCT cells compared to proliferative MCT cells. *: p<0.05; **: p<0.01. M: Marker.