Table 1.

Primers designed for real-time PCR

Name	RefSeqID	Sense Primer (5′-3′)	Antisense Primer (5′-3′)	Amplicon (bp)
Gapdh	NM_008084	ACCCAGAAGACTGTGGATGG	CACATTGGGGGTAGGAACAC	171
Col10α1	NM_009925	AAAGCTTACCCAGCAGTAGG	ACGTACTCAGAGGAGTAGAG	331
mTrp53	NM_001127233	TGGAAGACTCCAGTGGGAAC	CTGTAGCATGGGCATCCTTT	319
mTrp63	NM_001127259	GTCAGCCACCTGGACGTATT	CTCATTGAACTCACGGCTCA	222
mTrp73	NM_0011276331	CAAAGTGTCCACACCACCAC	CATACGGCACAACCACACTC	233
Bax	NM_0007527	TGCAGAGGATGATTGCTGAC	GATCAGCTCGGGCACTTTAG	173
Bcl-2	NM_009741	CTGGCATCTTCTCCTTCCAG	GACGGTAGCGACGAGAGAAG	183
Runx2	NM_001145920	ACCCAGC CACCTTTACCTAC	TATGGAGTGCTGCTGGTCTG	150
Sox9	NM_011448	TTCATGAAGATGACCGACGA	ATGCACACGGGGAACTTATC	200
Alp	NM_007431.2	AGTGAGCGCAGCCACAGAGC	GTGTGGCGTGGTTCACCCGA	134
Ank	NM_020332.4	CCTGGCCACGCAGCGAATCA	GACCCACGGGGTAGGTGGCT	119
Enpp1	NM_008813.3	CTCATGCCCCTCTGGGCGTC	CGGTGGCGTGAGGAACCCAT	168
Phospho1	NM_153104.3	GCTCTGGCTCCCCGGCATTTA	AGCCCCGAGGGACTGTGGATG	105

Gapdh: glyceraldehydes-3-phosphate dehydrogenase; Col10α1: type X collagen, alpha 1; mTrp53/63/73: murine transformation related protein 53/63/73; Bax: BCL-2-associated X protein; Bcl-2: B-cell leukemia (lymphoma) 2; Runx2: runt-related transcription factor 2; Sox9: SRY-box containing gene 9; Alp: Alkaline phosphatase; Ank: progressive ankylosis gene; Enpp1: ectonucleotide pyrophosphatase/phosphodiesterase 1; Phospho1: phosphatase, orphan 1