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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Neuropharmacology. 2012 Jan 2;62(5-6):1935–1943. doi: 10.1016/j.neuropharm.2011.12.026

Figure 2.

Figure 2

Representative superfusion scans illustrating the effect of the buffer switch protocol. Data shown is for [3H]-DA release from α6KO striatum. Units of release are net release as a percentage of baseline. The +Ca++ baseline is calculated as a single exponential decay from cpm collected in fractions 15-23. Open symbols represent only a buffer switch (□ with 300 nM nicotine in Ca++-free buffer, △ with no nicotine in Ca++-free buffer), a shift peak is seen which is not affected by the presence of nicotine in the Ca++-free buffer. The effect of nicotine treatment on stimulation by 10 μM ACh is shown by closed symbols (■ with nicotine exposure, and ▲ no nicotine exposure). As seen, nicotine treatment substantially decreases release promoted by ACh.