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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Nov 30;49(2):158–170. doi: 10.1016/j.mcn.2011.11.004

Figure 2. ATP activation of P2 purinergic receptors increases basal and apical cell proliferation in mouse OE in vivo.

Figure 2

Mice were intranasally instilled with saline vehicle (A–B) or P2 purinergic receptor antagonist, PPADS (50 nmoles/kg) and suramin (200 nmoles/kg), (C–D) 30 min prior to saline vehicle (A and C) or ATP (400 nmoles/kg, B and D) and tissue was collected 48 hours post-instillation of ATP. (A–D) Representative images of PCNA immunostaining. Dotted white line depicts the apical (AL), neuron (NL) and basal layer (BL). Arrows indicate PCNA+ cells in the apical layer. Scale bar = 5 μm. (E–F) Quantification of PCNA+ cells in the basal (E) and apical (F) layer of OE (n = 12 sections from 4 mice/group). * indicates significant differences from vehicle at p < 0.05. # indicates significant differences from ATP at p < 0.01 (two-way ANOVA followed by Newman-Keuls post hoc test).