Figure 8. NPY transiently activates p44/42 ERK at 5 min and FGF2 produces prolonged activation of p44/42 ERK in the OE in vitro.
OE primary cell cultures were incubated with saline vehicle (0 min), NPY (1 μM, A–C) or FGF2 (3 μM, D–F) for 5, 30 min and 1 hour. (A and D) Representative immunoblots for phospho-p44/42 ERK and actin. (B–C and E–F) Quantification of phospho-p44/42 ERK was made from 4 replications. The levels of phospho-p44/42 ERK were normalized to actin. Data are expressed as ratios over 0 min. * indicates significant differences from 0 min at p<0.05.