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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Vet Comp Oncol. 2011 May 18;10(1):44–56. doi: 10.1111/j.1476-5829.2011.00274.x

Figure 5. Addition of clodronate to vincristine treatment increased cell cycle arrest in canine MH cells.

Figure 5

The effect of the addition of clodronate to vincristine-treated DH82 cells was assessed using flow cytometry and cell cycle analysis. In (A), representative flow cytometry histograms are displayed for cells treated for 48 hours with clodronate (5 μg/mL), vincristine (0.2 μg/mL), or with both in combination. In cells treated with the combination of both drugs, there as a notable increase in the percentage of DH82 cells exhibiting G2/M arrest (R3 = G2/M). In (B), the mean percentages of cells in the G2/M stage of cell cycle progression were calculated for untreated cells or cells treated with clodronate or vincristine, alone or together. A significant increase (* = p < 0.05) in cells in the G2/M stage was observed in cells treated with clodronate plus vincristine, as assessed by ANOVA.