Figure 2. Identity of the epidermal cells that have incorporated FITC-DX probes.
BALB/c mice received s.c. injection of 70 kDa FITC-DX probes into ear skin. At 36 hours after the injection, the ear samples were harvested for immunofluorescence staining of the EDTA- separated epidermal sheets with AF546-conjugated mAb against Langerin (panel a) or against MHC class II molecules (panels b and c). Scale bar: 50 μm.