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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Microvasc Res. 2011 Nov 10;83(2):138–145. doi: 10.1016/j.mvr.2011.11.004

Figure 5.

Figure 5

Confluent brain microvascular endothelial cell cultures were subjected to hypoxia (1% O2) for various periods of time. Total RNA was extracted, reverse transcribed and amplified with gene specific primers for HO-1 (A); TSP-1 or TSP-2 (B). mRNA levels of HO-1, TSP-1, or TSP-2 were determined by normalizing their band densities to those of β-actin. Data in the bottom panel are the means ± SD of 3 experiments and expressed as percent of untreated control. 2HR: 2 h hypoxia followed by 2 h reoxygenation. **p<0.01, ***p<0.001 vs. 0 h.